Pitfall in HPLC assay for urinary metanephrines: an unusual type of interference caused by methenamine intake.

نویسندگان

  • Hanneke W M van Laarhoven
  • Jacques J Willemsen
  • H Alec Ross
  • Louk V A M Beex
  • Jacques W M Lenders
  • Fred C G J Sweep
چکیده

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Quantification of unconjugated metanephrines in human plasma without interference by acetaminophen.

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Freedom from drug interference in new immunoassays for urinary catecholamines and metanephrines.

BACKGROUND Determination of urinary free catecholamine and total (i. e., free plus conjugated) metanephrine excretion is considered the most clinically sensitive biochemical test for pheochromocytoma. In this study, we evaluated new immunoassay methods for the measurement of these analytes for potential drug-based interference. METHODS Urine samples collected from patients on a variety of med...

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BACKGROUND Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. METHODS Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine ...

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عنوان ژورنال:
  • Clinical chemistry

دوره 50 6  شماره 

صفحات  -

تاریخ انتشار 2004